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Do not place wires and other objects between the sealing surface and the door on the oven's front face. • The thin latex surgical vinyl and nitrile gloves that are popular in many laboratories may not be appropriate for use with highly toxic chemicals or solvents because of their composition and thin construction. Safety first model 2 scenes in the lab answers. 5 Safety Showers and Eyewash Units. The materials of construction (metal, elastomer, and plastic components) must be compatible with the gases and solvents being used. Install overhead racks and floor channel covers if wires must pass over or under walking areas.

Distillation of flammable and combustible solvents is dangerous due to the presence of heat and flammable vapors. • Vent pressure-relief devices that may discharge toxic, corrosive, flammable, or otherwise hazardous or noxious materials in a safe and environmentally acceptable manner such as scrubbing or diluting with nonflammable streams. 3 Eye and Face Protection. Model 2 scenes in the lab answer. The extreme cold of cryogenic liquids requires special care in their use. However, never use oil or lubricant on any equipment that will be used with oxygen.

The fly facility at the Francis Crick Institute looks after 1. Generally, select a gauge with a range that is double the working pressure of the system. This attitude begins with wearing appropriate apparel and using proper eye, face, hand, and foot protection when working with hazardous materials. Wear protective equipment in case a line or vessel that is opened contains material under pressure. Because significant breakthrough can occur at a fraction of the canister capacity, knowledge of the potential workplace exposure and length of time the respirator will be worn is important. The disconnect switch should automatically shut off the equipment when the top is opened. The lab desk will be hotWhat should you do if you need to place a hot apparatus on a lab desk? 147, Lock out/Tag out) applies. Convenient ways to check for leaks include a flammable gas leak detector (for flammable gases only) or looking for bubbles after application of soapy water or a 50% glycerin–water solution. Model 2 scenes in the lab answers. Dispose them in their proper containers, and not in the sinkWhat type of wastes do you dispose in the sink?

Thus, using a variable autotransformer that controls voltage and not frequency could cause the motor to overheat and presents a fire hazard. Never carry or move an evacuated desiccator. If trained laboratory personnel do undertake repairs, always unplug the cord before any disassembly begins. Heat these baths by an enclosed heating element, such as a knife heater, a tubular immersion heater such as a calrod, or its equivalent. Only qualified personnel wearing proper personal protective equipment (PPE) are allowed to open these boxes to access the main switches and circuit breakers contained therein. Perform leak checks periodically. Qualified personnel should conduct the replacement. Do not catch sharp instruments. As a result, the supplied air flows outward from the mask, and contaminated air from the work environment cannot readily enter the mask. Other physical hazards include electromagnetic radiation from lasers and radio-frequency generating devices. Fit laboratory equipment plugged into a 110-V (or higher) receptacle with a standard three-conductor line cord that provides an independent ground connection to the chassis of the apparatus (see Figure 7.

"You certainly don't have to be a scientist to play a part in life-changing scientific research. C. all of the above. Chemical splash goggles have splash-proof sides to fully protect the eyes. Vacuum work can be carried out on a Schlenk line, an apparatus used for work with air-sensitive compounds, as long as proper technique is used. • The maximum setting of a pressure-relief device is the rated maximum allowable working pressure (MAWP) established for the vessel or for the weakest member of the pressure system at the operating temperature. Thermal distillation uses an average of 70, 000 gal of water per coolant line, per year; the column purification system uses no water. Hand injuries are the most frequently encountered injuries in laboratories.

1 The outlet is always "female"; the plug is always "male. The electric heaters used to defrost the freezing coils are also a potential spark hazard (see section 7. Seal centrifuge bottles with rubber stoppers clamped in place, wrapped with friction tape and shielded with a metal screen or wrapped with friction tape and surrounded by multiple layers of loose cloth toweling, and clamped behind a good safety shield. Y/NNNever use ch---ed, cr---ed, or d---y ipped, cracked, dirtyAssume glassware is washed before you use it because the person who used it before you washed it for sure. • Locate electrical equipment to minimize the possibility of spills onto the equipment or flammable vapors carried into it.

• When bringing ordinary electrical equipment such as vacuum cleaners and portable electric drills having series-wound motors into the laboratory for special purposes, take specific precautions to ensure that no flammable vapors are present before such equipment is used (see Chapter 6, section 6. Do not use variable autotransformers to control the speed of an induction motor. Copper tubing hardens and cracks on repeated bending. The primary hazards of cryogenic liquids are frostbite, asphyxiation, fire or explosion, pressure buildup (either slowly or due to rapid conversion of the liquid to the gaseous state), and embrittlement of structural materials. • Use full-face shields with throat protection and safety glasses with side shields when handling explosive or highly hazardous chemicals. The material should not react with the process being studied, and the vessel must be of the proper size and configuration. Texture Resolutions: 16-16, 256-256. Erasure of credit card and bank cards; disruption of small mechanical devices, such as analog watches and clocks; and disturbance of X-ray tubes. Use them for immediate first-aid treatment of chemical splashes and for extinguishing clothing fires. However, a modified spark-proof refrigerator cannot meet the standards of an explosion-proof refrigerator.

Understandably, injuries to the hands are very common in the laboratory. This overload protection is particularly useful for equipment likely to be left on and unattended for a long time, such as variable autotransformers (e. g., Variacs and powerstats), 2 vacuum pumps, drying ovens, stirring motors, and electronic instruments. Lab workers had left samples running unattended in an ultracentrifuge using a large aluminum rotor that previously had been used multiple times without incident. They should be performed as infrequently as possible but before the vessel is placed into initial service, every 10 years thereafter, after a significant repair or modification, and if the vessel experiences overpressure or overtemperature. See, e. g., Yarnell (2002). Molten salt baths, like hot oil baths, offer the advantages of good heat transfer, commonly have a higher operating range (e. g., 200 to 425 °C), and may have a high thermal stability (e. g., 540 °C). Machine warning lights indicate when the beam shutter is open. Higher voltages will cause a mantle to overheat, melting the fiberglass insulation and exposing the bare heating element. Because superconducting magnets use liquid nitrogen and liquid helium coolants, the precautions associated with the use of cryogenic liquids must be observed as well. ) 132) and the OSHA Laboratory Standard (29 CFR § 1910.

Under most circumstances, any repairs to, adjustments to, or alterations of electrical instruments should be made only by a qualified individual. An eyewash unit provides a soft stream or spray of aerated water for an extended period (15 minutes). Some bottles are typically equipped with a head-containing inlet and exhaust gas valves, a pressure gauge, and a pressure-relief valve. As biomedical research advances, the tiny fruit fly Drosophila remains a key model organism used by biomedical researchers – not least because 70% of genes linked to human disease have a fruit-fly equivalent. These 'cell lines' – cells that can reproduce indefinitely – have to be cultured and then frozen for storage in the cell line repository. Handle objects that are in contact with cryogenic liquids with tongs or potholders. Always keep connections to piping, regulators, and other appliances tight to prevent leakage, and keep the tubing or hoses used in good condition. The design shown is for 15-A, 125-V service.

Do not use an adapter or cross-thread a valve fitting. Create an account to follow your favorite communities and start taking part in conversations. However, certain adjustments require connection to a power source, and appropriate protective measures and due diligence are required when working on energized devices. • If electrical equipment must be placed in such areas, mount the equipment on a wall or vertical panel. As previously noted, use only hot plates that have completely enclosed heating elements in laboratories.

Most thermal stills rely on electric heating mantles to heat the flammable solvents upward of 82 °C (180 °F), presenting a fire risk and potential ignition source. Glass components of the rotary evaporator should be made of Pyrex or similar glass. Masks come in different sizes and cannot be considered universal or one-size-fits-all. Tubing and piping downstream of such devices must be at least the same diameter as the fitting on the vent side of the relief device. Microwave ovens can heat material (e. g., solidified agar) so quickly that, even though the container lid is loosened to accommodate expansion, the lid can seat upward against the threads and the container can explode. Independent temperature sensors must be used for the temperature controller and shutoff devices. Second, the amount of organic matter placed in the furnace in the form of the polypropylene samples was significantly more than that in the usual paint samples.

For many experiments, extremes of both pressure and temperature, such as reactions at elevated temperatures and pressures and work with cryogenic liquids and high vacuum, must be managed simultaneously. However, in practice, such modifications are difficult to incorporate into many of the hot plates now in use. Personal clothing should fully cover the body. This incident highlights the importance of deenergizing systems and processes prior to disassembly or maintenance. The event of breakage. Let only qualified individuals service electrical equipment.

Other types of extinguishers should be available if required for the work that will be performed in the laboratory. Number of Textures: 2. Regular inspection of their serviceability is important. Reinforced plastic tubing that can withstand higher pressures is also available. Electrically heated ovens are commonly used in the laboratory to remove water or other solvents from chemical samples and to dry laboratory glassware. Do not control the speed of an induction motor operating under a load by a variable autotransformer. Additional protection may be required if the airborne contaminant could be absorbed through or irritate the skin. 2 Commonly known as "variacs, " variable autotransformers are devices that provide a voltage-adjustable output of AC electricity using a constant voltage input (e. g., the wall outlet).

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